Geobacter Project University of Massachusetts Geobacter Project

Microbial Fuel Cells

We have found that dissimilatory metal-reducing microorganisms, such as Geobacter and Rhodoferax species, have the novel ability to directly transfer electrons to the surface of electrodes. This had led to the construction of microbial fuel cells that are superior to previously described microbial fuel cells in that: 1) they are much more efficient; 2) they do not require the addition of the toxic electron shuttling mediator compounds employed in previously described microbial fuel cells; 3) they have remarkable long-term stability; and 4) it is possible to harvest electricity from many types of waste organic matter or renewable biomass. Immediate application of these microbial fuel cells will be for powering electronic monitoring devices in remote locations, such as the bottom of the ocean. However, many other applications are possible. Current research is focusing on elucidating the mechanisms of electron transfer between the microorganisms and the electrode in order to design better electrodes or genetically engineer better microbes for higher rates of electricity production.

[Sediment Battery Preparation | .pdf (177 KB)]

References

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Izallalen, M., R. Mahadevan, A. Burgard, B. Postier, R. DiDonato, J. Sun, C.H. Schilling, and D.R. Lovley. 2008. Geobacter sulfurreducens strain engineered for increased rates of respiration. Metab Eng 10(5):267-275.

Nevin, K.P., H. Richter, S.F. Covalla, J.P. Johnson, T.L. Woodard, A.L. Orloff, H. Jia, M. Zhang, and D.R. Lovley. 2008. Power output and columbic efficiencies from biofilms of Geobacter sulfurreducens comparable to mixed community microbial fuel cells. Environ Microbiol [epub ahead of print]

Kim, B.-C. , B.L. Postier, R.J. DiDonato, S.K. Chaudhuri, K.P. Nevin, and D.R. Lovley. 2008. Insights into genes involved in electricity generation in Geobacter sulfurreducens via whole genome microarray analysis of the OmcF-deficient mutant. Bioelectrochemistry 73(1):70-75.

Lovley, D.R. 2008. Extracellular electron transfer: wires, capacitors, iron lungs, and more. Geobiology 6(3):225-231.

Lovley, D.R., and K.P. Nevin. 2008. Chapter 23: Electricity production with electricigens. In J. Wall et al. (ed.), Bioenergy. ASM Press, Washington, DC. pp. 295-306.

Holmes, D.E., T. Mester, R.A. O'Neil, L.A. Perpetua, M.J. Larrahondo, R. Glaven, M.L. Sharma, J.E. Ward, K.P. Nevin, and D.R. Lovley. 2008. Genes for two multicopper proteins required for Fe(III) oxide reduction in Geobacter sulfurreducens have different expression patterns both in the subsurface and on energy-harvesting electrodes. Microbiology(154):1422-1435.

Richter, H., K. McCarthy, K.P. Nevin, J.P. Johnson, V.M. Rotello, and D.R. Lovley. 2008. Electricity generation by Geobacter sulfurreducens attached to gold electrodes. Langmuir 24(8):4376-4379.

Lanthier, M., K.B. Gregory, and D.R. Lovley. 2008. Growth with high planktonic biomass in Shewanella oneidensis fuel cells. FEMS Microbiol Lett 278(1):29-35.

Richter, H., M. Lanthier, K.P. Nevin, and D.R. Lovley. 2007. Lack of electricity production by Pelobacter carbinolicus indicates that the capacity for Fe(III) oxide reduction does not necessarily confer electron transfer ability to fuel cell anodes. Appl Environ Microbiol 73(16):5347-5353.

Holmes, D.E., K.P. Nevin, T.L. Woodard, A.D. Peacock, and D.R. Lovley. 2007. Prolixibacter bellariivorans gen. nov., sp. nov., a sugar-fermenting, psychrotolerant anaerobe of the phylum Bacteroidetes, isolated from a marine-sediment fuel cell. Int J Syst Evol Microbiol 57(Pt 4):701-707.

Reguera, G., K.P. Nevin, J.S. Nicoll, S.F. Covalla, T.L. Woodard, and D.R. Lovley. 2006. Biofilm and nanowire production leads to increased current in Geobacter sulfurreducens fuel cells. Appl Environ Microbiol 72(11):7345-7348.

Holmes, D.E., S.K. Chaudhuri, K.P. Nevin, T. Mehta, B.A. Methé, A. Liu, J.E. Ward, T.L. Woodard, J. Webster, and D.R. Lovley. 2006. Microarray and genetic analysis of electron transfer electrodes in Geobacter sulfurreducens. Environmental Microbiology 8(10):1805-1815.

Lovley, D.R. 2006. Microbial energizers: Fuel cells that keep on going. Microbe 1(7):323-329.

Lovley, D.R. 2006. Taming electricigens: How electricity-generating microbes can keep going, and going — faster. The Scientist 7(20):46.

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Lowy, D.A., L.M. Tender, J.G. Zeikus, D.H. Park, and D.R. Lovley. 2006. Harvesting energy from the marine sediment-water interface II: Kinetic activity of anode materials. Biosens Bioelectron 21(11):2058-2063.

Gregory, K.B., and D.R. Lovley 2005. Remediation and recovery of uranium from contaminated subsurface environments with electrodes. Environ Sci Technol 39(22):8943-8947.

Holmes, D.E., K.P. Nevin, R.A. O'Neil, J.E. Ward, L.A. Adams, T.L. Woodard, H.A. Vrionis, and D.R. Lovley. 2005. Potential for quantifying expression of the Geobacteraceae citrate synthase gene to assess the activity of Geobacteraceae in the subsurface and on current-harvesting electrodes. Appl Environ Microbiol 71(11):6870-6877.

Bond, D.R., and D.R. Lovley. 2005. Evidence for involvement of an electron shuttle in electricity generation by Geothrix fermentans. Appl Environ Microbiol 71(4):2186-2189.

Holmes, D.E., D.R. Bond, R.A. O'Neil, C.E. Reimers, L.R. Tender, and D.R. Lovley. 2004. Microbial communities associated with electrodes harvesting electricity from a variety of aquatic sediments. Microb Ecol 48(2):178-190.

Holmes, D.E., J.S. Nicoll, D.R. Bond, and D.R. Lovley. 2004. Potential role of a novel psychrotolerant member of the family Geobacteraceae, Geopsychrobacter electrodiphilus gen. nov., sp. nov., in electricity production by a marine sediment fuel cell. Appl Environ Microbiol 70(10):6023-6030.

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Videos
Bug Powered Batteries (PBS Science Investigators)

Photos (click on thumbnail for full size image)

Dr. Sarah Strycharz with marine sediment fuel cell powered robot

Dr. Ashley Franks demonstrates toy bat powered by marine sediment fuel cell

Sean Covalla with the electric bat

Jenny Richards takes a measurement of electrodes in an artificial rice paddy

Dr. Kelly Nevin takes an H-cell out for analysis

Dr. Kelly Nevin takes a reading from a mini-stack

Prof. Derek Lovely with a marine sediment fuel cell

Marine sediment fuel cell powered toy SUV makes tracks
Press Releases, Wire Stories, and Media Coverage

2007-01-10: Bug Powered Batteries (PBS Science Investigators)

2004-04-22: Mud Is Making Waves - Electrical Ones (Chronicle, WCVB-TV5)

2003-12-16: Bacterial Batteries (Real Media) (ScienCentral.com)

2003-12-16: Bacterial Batteries (QuickTime) (ScienCentral.com)

2007-06-01: Microbes Power Up (MicrobeWorld Radio)

2005-06-22: Radio spot on Geobacter, electricity production, and nanowires (WFCR FM-88.5)

2004-05-20: Geobacter: Creating Electricity From Mud (Pulse of the Planet)

2004-03-14: Electricity from Mud (WTOP News on Engineering)

2003-10-08: Bacterial Batteries (Science Update)

2007-12-06: Bacterias generadoras de electricidad (El Faro (UNAM))

2007-08-30: Green Energy: Eco-Friendly Batteries (Time Magazine)

2007-05-15: Harnessing the Power of Microbes (BioCycle, May 2007, Vol. 48, No. 5)

2007-05-15: Power In Space: Time For A Biological Solution (Space Daily)

2006-06-06: Fuel cell power (FuelCellWorks)

2006-06-05: The Tiniest Power Plants: Scientists are seeing a host of possibilities in electricity produced by microbes (Business Week)


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