FN ISI Export Format
VR 1.0
FN ISI Export Format
VR 1.0
PT J
AN 19921843
DT Journal Article; Research Support, Non-U.S. Gov't
TI Electrode-based approach for monitoring in situ microbial activity during subsurface bioremediation.
AU Williams, Kenneth H
   Nevin, Kelly P
   Franks, Ashley
   Englert, Andreas
   Long, Philip E
   Lovley, Derek R
SO Environ Sci Technol
VL 44
IS 1
PS 47-54
PY 2010
PD 2010 Jan 1
LA English
AB Current production by microorganisms colonizing subsurface electrodes
   and its relationship to substrate availability and microbial activity
   was evaluated in an aquifer undergoing bioremediation. Borehole
   graphite anodes were installed downgradient from a region of acetate
   injection designed to stimulate bioreduction of U(VI); cathodes
   consisted of graphite electrodes embedded at the ground surface.
   Significant increases in current density (< or =50 mA/m2) tracked
   delivery of acetate to the electrodes, dropping rapidly when acetate
   inputs were discontinued. An upgradient control electrode not exposed
   to acetate produced low, steady currents (< or =0.2 mA/m2). Elevated
   current was strongly correlated with uranium removal but minimal
   correlation existed with elevated Fe(II). Confocal laser scanning
   microscopy of electrodes revealed firmly attached biofilms, and
   analysis of 16S rRNA gene sequences indicated the electrode surfaces
   were dominated (67-80%) by Geobacter species. This is the first
   demonstration that electrodes can produce readily detectable currents
   despite long-range (6 m) separation of anode and cathode, and these
   results suggest that oxidation of acetate coupled to electron transfer
   to electrodes by Geobacter species was the primary source of current.
   Thus it is expected that current production may serve as an effective
   proxy for monitoring in situ microbial activity in a variety of
   subsurface anoxic environments. 
C1 Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA. khwilliams@lbl.gov
SS Index Medicus
SN 0013-936X
JC 0213155
PA United States
RC 30 Dec 2009
UT MEDLINE:19921843
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