FN ISI Export Format VR 1.0 FN ISI Export Format VR 1.0 PT J AU Qu, YH Brown, P Barbe, JF Puljic, M Merino, E Adkins, RM Lovley, DR Krushkal, J AF Qu, Yanhua Brown, Peter Barbe, Jose F. Puljic, Marko Merino, Enrique Adkins, Ronald M. Lovley, Derek R. Krushkal, Julia TI GSEL Version 2, an Online Genome-Wide Query System of Operon Organization and Regulatory Sequence Elements of Geobacter sulfurreducens SO OMICS-A JOURNAL OF INTEGRATIVE BIOLOGY LA English DT Article ID URANIUM-CONTAMINATED AQUIFER; ESCHERICHIA-COLI K-12; FE(III) REDUCTION; COMMUNITY STRUCTURE; ELECTRON-TRANSFER; METAL REDUCTION; GENE-REGULATION; COG DATABASE; DNA; TRANSCRIPTION AB Geobacter sulfurreducens is a model organism within the delta-Proteobacterial family Geobacteraceae, members of which can participate in environmental bioremediation of metal and organic waste contaminants and in production of bioenergy. In this report, we describe a new, significantly expanded and updated, version 2 of the GSEL (Geobacter Sequence Elements) database (http://geobacter.org/research/gsel2/ and http://geobacter.org/refs/gsel2/) and its accompanying online query system, which compiles information on operon organization and regulatory sequence elements in the genome of G. sulfurreducens. It incorporates a new online graphical browser, provides novel search capabilities, and includes updated operon predictions along with new information on predicted and experimentally validated genome regulatory sites. The GSEL database and online search system provides a unique and comprehensive tool cataloging information about gene regulation in G. sulfurreducens, aiding in investigation of mechanisms that regulate its ability to generate electric power, bioremediate environmental waste, and adapt to environmental changes. C1 [Qu, Yanhua; Barbe, Jose F.; Puljic, Marko; Krushkal, Julia] Univ Tennessee, Hlth Sci Ctr, Dept Prevent Med, Memphis, TN 38163 USA. [Brown, Peter; Lovley, Derek R.] Univ Massachusetts, Dept Microbiol, Amherst, MA 01003 USA. [Puljic, Marko] Univ Memphis, Dept Math Sci, Memphis, TN 38152 USA. [Merino, Enrique] Univ Tennessee, Hlth Sci Ctr, Dept Pediat, Memphis, TN 38163 USA. RP Krushkal, J, Univ Tennessee, Hlth Sci Ctr, Dept Prevent Med, 66 N Pauline,Ste 633, Memphis, TN 38163 USA. EM jkrushka@utmem.edu FU Office of Science (BER) ; U. S. Department of Energy [DE-FC02-02ER63446] FX This research was supported by the Office of Science (BER), U. S. Department of Energy, Grant No. DE-FC02-02ER63446. We thank two anonymous reviewers and Dr. E. Kolker, the editor of OMICS, for helpful comments and suggestions. We are grateful to Drs. V. Solovyev (Softberry, Inc) and A. Salamov (Lawrence Berkeley National Laboratory) for information about algorithms implemented in the FGENESB software and for helpful discussions. We also thank J. Peeples (the University of Tennessee Health Science Center) for editorial assistance. NR 50 TC 0 PU MARY ANN LIEBERT INC PI NEW ROCHELLE PA 140 HUGUENOT STREET, 3RD FL, NEW ROCHELLE, NY 10801 USA SN 1536-2310 J9 OMICS JI OMICS PD OCT PY 2009 VL 13 IS 5 BP 439 EP 449 DI 10.1089/omi.2009.0081 PG 11 SC Biotechnology & Applied Microbiology; Genetics & Heredity GA 500TO UT ISI:000270328100008 ER EF EF